For n2 limited cultures, nan03 was replaced by kc1, and nitrate ammonia, and urea were tested in different concentrations as. Spirulina medium recipe directions modification of the sag medium. Microalgae strain catalogue a strain selection guide for microalgae users. A comparison of growth in terms of change in turbidity 750 nm after 5 days indicated that there was about 250% change in turbidity of the cultures grown in zarrouk s medium. Spirulina culture medium list with recipes and suppliers. The culture broth was a mixture of ricotta whey diluted with zarrouk medium zarrouk, 1966, in ratio 1. Two of them contained a high percentage of protein 68. This study assessed the biomass, proximate composition, and other useful compounds in spirulina arthrospira fusiformis produced with a costeffective culture medium lcma, and the results were compared with those from a standard zarrouk mediumgrown spirulina. Utilization of co2 in semicontinuous cultivation of spirulina sp. These acidic liquids require special care, as they involve the use of hazardous acids which, if not handled properly, can cause serious damage and discomfort, irritating the eyes and skin of the preparer.
Effect of modified zarrouks medium on growth of different. Materials and methods microalgae the strain of arthrospira fusiformis used in this study was obtained from the stock culture kept at the institute of marine sciences, university of dar es salaam, tanzania. The respective growth was monitored and contents were tested. Table 1 chemical composition of zarrouks medium and the new reduced cost media. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. Formulation of costeffective medium using urea as a nitrogen source for arthrospira platensis cultivation under real environment. Influence of growth medium composition on synthesis of bioactive. Optimization of spirulina platensis biomass and evaluation. One liter of zarrouks medium consists of part a nahco3 16. In order to prevent the formation of precipitates during autoclaving, two solutions are prepared as follows. Zarrouk s synthetic solid medium zarrouk, 1966,amended with 2%wv agar was used for maintaining s. Allow to cool then store at refrigerator temperature. Biomass concentration as dry weight and protein content of cultivation in complex medium were lower to those observed in the inorganic zarrouk medium, regardless of the light intensity.
Spirulina has been cultivated commercially for their valuable natural pigments and also used as dietary supplements. Further study is required with a focus on one or two particular nutrients with alteration in concentration in one type of medium, and other. The strain was maintained in a modified zarrouk s medium9 in 500ml flasks containing 200ml of culture at room temperature. During our testing of media compositions using chlorella and arthrospira both. To determine the optimal temperature for growth, cultures were incubated at four different temper. Chemical composition and potential application of spirulina platensis. Optimization of biomass production of spirulina platensis in. As most pathogenic microbes require complex food similar in composition. It is a challenge for the researchers to develop a low cost medium for the mass production of spirulina. Zarrouk s medium and compare the specific growth rate, doubling time, mean daily division rate, to biomass, and chlorophylla.
Effects of medium composition, calcium, iron and oxygen on haemolysin production by plesiomonas shigelloides isolated from water k. Ast medium at 1x concentration is similar to zarrouks medium, while 2x medium contains a high amount of salt. Zarrouk medium, bg11 medium, conway medium, f2 medium and seawater medium for thirty days. Pdf effect of modified zarrouks medium on growth of different. Minimising the cost of spirulina platensis culture medium. Mar 16, 2008 the ingredient of zarrouk culture medium. The authors also observed the maximum content of phycoerythrin in synthetic human urine shu medium. Growth performance of spirulina arthrospira platensis in. Optimization of arthrospira platensis spirulina growth.
Short communication formulation of organic medium for the. Therefore, the second step of the study was conducted for scaleup based on the highest biomass production with the cheapest medium, which was the m19 medium. Cultivation of spirulina at commercialscales relies on analytical gradebased media, which are expensive and so are the product. Dec 21, 2005 human urine contains minimal organic matters, but much inorganic matter, such as n, p and k larsen et al.
Beijerinck medium composition for culturing of algae in laboratory of botany and plant science by. This intention was implemented by substituting all the nutrients present in zarrouk s medium sm with cheaper and locally available commercial fertilizers and chemicals. The effect of modified zarrouks medium on the growth response of 6 different spirulina strains was evaluated. Pdf evaluation of media composition on the production of c. How to make nasas solution b6 for zarrouk growth medium 1966. The results show that the cost of preparing the culture medium using vinh hao mineral water is less than 252.
This study assessed the biomass, proximate composition, and other useful compounds in spirulina arthrospira fusiformis produced with a costeffective culture medium lcma, and the results were compared with those from a standard zarrouk medium grown. Subsequently, different media have been tried for cultivation of spirulina such as revised medium raoof et al. Spirulina maxima was grown outdoors for 2 weeks in a 10l transparent plastic jar with two replications r1 and r2. Partial substitution of synthetic 25 zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content 4558%26 dry weight and favourable fatty acids composition 27 4245% pufas of total fatty acids. Variation of spirulina maxima biomass production in. Spirulina platensis was grown in zarrouk s medium with the initial medium ph 8. Growth medium screening for chlorella vulgaris growth and. The bicarbonate solutions were sterilized by filtration through 0. A study showed that zarrouks medium zm was the best medium in terms of biomass productivity, while modi. Here is the composition of the zarrouk medium, that i recommend to use for your tank at home. Isolation and characterization of a new cyanobacterial strain. For formulating a new and costeffective medium, in the first step, each of the individual constituents was substituted one by one after single super phosphate ssp supplying cah 2 po 4 2 caso 4 such that potassium and phosphorus equivalent to the amount present in the sm was provided and with muriate of potash.
The cultivations were biomass production by arthrospira platensis under different culture conditions. The effect of modified zarrouk s medium on the growth response of 6 different spirulina strains was evaluated. Zarrouk growth media spirulina nutrient mix grows 100. A study showed that zarrouk s medium zm was the best medium in terms of biomass productivity, while modi. Read the effect of low light flux and nitrogen deficiency on the chemical composition of spirulina sp. Formulation of a lowcost medium for mass production of spirulina. Cultures are tested under both n sufficiency and n limitation and the effects of co 2.
Optimization of growth of spirulina platensis ln1 for production of carotenoids m thirumala research paper growth of spirulina platensis ln1 was observed using eight different media consisting of different combinations of lonar lake water, n, p and k and zarrouk medium composition under different incubation periods. The first synthetic medium formulated for cultivation of spirulina was zarrouks medium zarrouk, 1966 which is still used as the standard medium. This kit is a premixed formula of all necessary nutrients and minerals to grow 100 gallons of spirulina growth media. Specific growth rate, doubling time, mean daily division rate, biomass, and. We reported here our attempts at culturing spirulina platensis in human urine solely and directly to achieve nutrient regeneration and air revitalization. Cells were grown under continuous illumination with white luminescent lamps at 110 moles of photons m.
In this study, organic medium used and growth performance of spirulina was studied based on th. Isolation and characterization of a new cyanobacterial. Cultivation of spirulina platensis in different selective media. Pdf effect of modified zarrouks medium on growth of. Research article new medium for pharmaceutical grade.
Biomass and nutritive value of spirulina arthrospira. Cultivation of spirulina platensis in different selective. Directions in order to prevent the formation of precipitates during autoclaving, two solutions are prepared as follows. Zarrouk s medium was used as the sm, and its composition is given in table 1. The biomass, ph, and salinity were measured during inoculation and at the end of the study. Alkaline media suitable for lb 2340 spirulina platensis and lb 2342 spirulina maxima directions. Production of carbohydrates depends on composition of medium and its ph. The concentration of the original formulation, termed f medium guillard and ryther 1962, has been reduced by half. Jul 11, 2012 the culture medium or the water in which spirulina grows is one of the most import parameter when growing spirulina at home. Although alterations were made in the basic composition, the media so developed commercially were. Formulation of a lowcost medium for mass production of. Our new medium has the ability to inhibit different forms of cyanobacterium and microalgae except the chlorella. Research article new medium for pharmaceutical grade arthrospira.
Zarrouks medium and compare the specific growth rate, doubling time, mean daily division rate, to biomass, and chlorophylla. Assessment of growth, production and nutritional composition of spirulina platensis under induced conditions of light regime. Dec 23, 2019 cultivation of spirulina at commercialscales relies on analytical gradebased media, which are expensive and so are the product. Research article new medium for pharmaceutical grade arthrospira amroa.
Arthrospira grown on digested pig waste, bioresource technology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Zarrouk medium which is rich in bicarbonate has successfully served as a common culture medium in spirulina culture for years 2. Composition of the culture media used in the experiments. Despite having started with a similar initial inoculum, after 9 days of cultivation, the growth on desalinator wastewater was more intense than in the other two media. Partial substitution of synthetic zarrouk medium with 25% of wastewater showed no adverse effect on chemical composition of the biomass including high protein content 4558% dry weight and favorable fatty acid composition 4245% pufas of total fatty acids. After both solutions 1 and 2 are sterilized, transfer the contents. In zarrouk medium, bg11 medium and seawater medium, the ph becomes more basic. Pes medium as table 1 in which the salinity of the seawater was reduced to 20.
Assessment of growth, production and nutritional composition. This study aimed to provide a cost effective medium to large scale production of spirulina platensis. Assay 1 2 3 concentration of carbon source g l1 nahco. Production of pigments depends on composition of medium and its ph. Chlorophyll production from spirulina platensis single. Zarrouk s nutritive medium zarrouk, 1966 were used as starter inocula. Production and nutritive value of spirulina platensis in reduced cost.
Dec 12, 2016 the original 1966 zarrouk culture medium formula used by nasa included 2 liquid solutions. Cultivation of two species of spirulina spirulina platensis. Pdf formulation of costeffective medium using urea as a. A comparison of growth in terms of change in turbidity 750 nm after 5 days indicated that there was about 250% change in turbidity of the cultures grown in zarrouks medium. Six different spirulina strains were obtained from different sources table 1. The culture medium was modified for nutrient limitation studies.
For that, a mixed culture of pure chlorella and arthrospira. Four different types of spirulina biomass with a different chemical composition were tested. The composition of the medium was per liter of distilled water. Leb18 culture using effluent from the anaerobic dige stion 279 brazilian journal of chemical engineering vol. Photosynthesis, growth and cell composition of spirulina. Chemical composition of zarrouks medium and the new reduced cost media. New medium for pharmaceutical grade arthrospira hindawi. Pef medium in which the nutrients were the same as the pes medium, but freshwater was used instead of seawater.
The medium was autoclaved without the bicarbonate salts. For 1 l total after both solutions 1 and 2 are sterilized, transfer the contents of. Effects of medium composition, calcium, iron and oxygen on. Because spirulina will absorb whatever is in its environment, which is, you guested it, the culture medium. Journal of algal biomass utilization jabu is published by phycospectrum online launched in 2009 and updated monthly. The culture media nutrients consist of chemicals which support the growth of culture or microorganisms. Further study is required with a focus on one or two particular nutrients with alteration in concentration in one type of medium, and other factors remaining unchanged each time to find out the. Cultivation of spirulina platensis in different selective media nopr. The effects of media composition on the production of cphycocyanin cpc from the bluegreen algae spirulina platensis and the anti oxidant potential of the synthesizedcpc have been studied. The aim of this study is to understand the growth response of different strains under modified. However, discarding zarrouk medium with nitrate can also be a. Murashige and skoog basal medium catalog number m5519 storage temperature 28 c product description classic plant cell culture medium containing macro and micronutrients, and vitamins.
Growth performance of spirulina arthrospira platensis in a. Chemical composition and potential application of spirulina. The aim of this study is to produce a pharmaceutical grade single cell product of arthrospira from a mixed culture. Optimization of spirulina platensis biomass and evaluation of. This intention was implemented by substituting all the nutrients present in zarrouks medium sm with cheaper and locally available commercial fertilizers and chemicals. Zarrouk growth media is a popular combination of salts and minerals used in spirulina cultivation as the building blocks that help spirulina grow and thrive.
Biosorption of heavy metals influenced by the chemical. Vonshak1986 used to maintain the unicellular green algae. Production and nutritive value of spirulina platensis in. Zarrouk s medium formulated for spirulina cultivation as the standard medium sm. Biomass composition of arthrospira platensis during. Alkaline media suitable for lb 2340 spirulina platensis and lb 2342 spirulina maxima. Pdf production and nutritive value of spirulina platensis in. Spirulina strain and growth medium the spirulina platensis was obtained from department of microbiology, annamalai university and named as s5. We have designed a medium derived from a combination between georges and zarrouk s media.